Signal Transduction Reagents and Kits
Promega CDK2/CyclinA2 Kinase Enzyme System, 1mg
Recombinant full-length human CDK2 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed.
Promega ERK1 Kinase Enzyme System, 10microg
Recombinant full-length, tag-free human ERK1 was expressed by baculovirus in Sf9 insect cells and activated by active MEK1 in vitro. ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).
![Promega PI3K (p110alpha[H1047R]/p85alpha), 20ug, 200microl](https://assets.fishersci.com/TFS-Assets/CCG/product-images/PRV1741.jpg-250.jpg)
Promega PI3K (p110alpha[H1047R]/p85alpha), 20ug, 200microl
The PI3K lipid kinase enzymes are used with ADP-Glo KInase Assay System and Lipid Kinase Substrates in a homogeneous, add-mix-read lipid kinase assay.
Promega ERK2 Kinase Enzyme System, 1mg
Recombinant full-length human ERK2 was expressed by E. coli cells using an N-terminal GST tag and activated by MEK1 in vitro. ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).
Promega ADP-Glo™ Kinase Assay + ALK4 Kinase Enzyme System
Recombinant human ALK4 (150-end) was expressed in Sf9 cells using an N-terminal GST tag. ALK4 is a member of the subfamily of receptor ser/thr kinases that mediates signaling by the Activins.
Promega CDK5/p25 Kinase Enzyme System, 1mg
Recombinant full-length human CDK5 and p25 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain.
Promega ADP-Glo™ Kinase Assay + PYK2 Kinase Enzyme System
Recombinant human PYK2 (360-690) was expressed by baculovirus in Sf9 cells using an N-terminal His tag. PYK2 (also known as FAK2/RAFTK) is a member of the focal adhesion PTK family.
Promega ADP-Glo™ Kinase Assay + CDK2/CyclinE1 Kinase Enzyme System
Recombinant full-length human CDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed.
Promega ADP-Glo™ Kinase Assay + NEK3 Kinase Enzyme System
Recombinant full-length human NEK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK3 is a member of the NEK family of protein kinases that share high amino acid homology with NIMA (never in mitosis gene a).
Promega SIK Kinase Enzyme System, 10microg
Recombinant human SIK (1-303) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. SIK is a protein kinase that is involved in regulating AMPK-related kinases.
Promega Kinase Selectivity Profiling System: CAMK-1, 8 x 50 reactions
Easy-to-use kits for performing kinase selectivity profiling that rely on the ADP-Glo Kinase Assay technology. Each system includes kinase and substrate pairs in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.
Promega ADP-Glo™ Kinase Assay + TOPK Kinase Enzyme System
Recombinant full-length human TOPK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TOPK is a MAPK kinase that phosphorylates p38 MAPK and is activated in a cell-cycle-dependent manner in neuronal progenitor cells in vitro.
Promega ADP-Glo™ Kinase Assay + MST1 Kinase Enzyme System
Recombinant full-length human MST1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MST1 belongs to a family of proteins that share similarity with a budding yeast serine/threonine kinase, sterile-20 (Ste20).
Promega ADP-Glo™ Kinase Assay + PLK1 Kinase Enzyme System
Recombinant full-length human PLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. A member of the Polo-Like Kinase family, PLK1 localizes to centrosomes and undergoes dramatic subcellular relocation during the cell cycle.
Promega ADP-Glo™ Kinase Assay + TAOK1 Kinase Enzyme System
TAOK1 is a serine/threonine-protein kinase involved in regulation of the p38-containing stress-responsive MAP kinase pathway and extracellular signal-regulated protein kinase (ERK) kinases (MEKs).